Keywords : Male rats

The effect of diazepam in some fertility and testosterone levels in healthy adult male rats

Hadeel Mohammed Hamed

Journal of Education and Science, 2011, Volume 24, Issue 1, Pages 66-79
DOI: 10.33899/edusj.1999.51462

The present investigation was conducted to reveal the effect of oral administration of diazepam (2.5-5 mg/kg B.W.) orally daily for 6 weeks on sperm concentration, the level of live sperm, dead and abnormal sperm and the relative weight of testis, prostate, seminal vesicles and epidydimus (head, body, tail). Furthermore measurement of testosterone hormone level in time interval (0,3,6 weeks) in adult albino male rats with age between 2.5-3 months and average body weight (222±16.79gram). The results showed that diazepam at doses (2.5-5 mg/kg) body weight caused a significant decreas in sperms count and the percentage of live sperms with a significant increased in the percentage of dead sperms and morphologically abnormal sperms.
Furthermore a significant decreas in serum testosterone, average weight of testis, prostate glands and tail of epididymis associated with significant increased in average body weight during period 2,4,5,6, weeks in rats treated with diazepam at two doses 2.5,5 mg/kg of body weight compared with control group. It is concluded from the present study is that diazepam has adverse effect on sexual efficiency in adult male.

Effect of Apis mellifera toxin in the nervous system of healthy adult male rats treated with hydrogen peroxide

Karam Hashim Almallah; Muzahim Ayoob Alsaegh; Intesar Mansour Abdulrasool

Journal of Education and Science, 2010, Volume 23, Issue 4, Pages 75-92
DOI: 10.33899/edusj.2010.58461

The study was designed to demonstrate the effects of honey bee
(Apis mellifera) venom on the nervous system of normal and hydrogen peroxide treated male rats. Twenty four male rats were randomly divided to 4 groups, with 6 animals for each group: group 1 (control), group 2 (treated with 1% hydrogen peroxide with drinking water), group 3 (exposed to bee venom by normal stings according to 155 sting program) and group 4 (treated with hydrogen peroxide and exposed to stings). The groups treated for 49 days included 4 stinging periods, central nervous system and autonomic nervous system activities were monitored at the end of each period. The animals were sacrificed at the end of experiment period, Gross and histopathological examinations of the brain were performed. The result of the tests (moving oncet, open field, negative geotaxis) showed a significant decrease of central nervous system activities at the 3 treated groups from control with the progression of the experiment, lower levels were recorded at group 4. At the same time there were no expressional significant deferences appeared at sensomobilary stimulatory response tests including (approach, touch, sound, tail pinching) tests reflecting activity of autonomic nervous system. The histopathological examination of brain revealed pathological changes at treated groups represented by vaculation of brain nervous cells, infiltration of microgelial cells, congestion of capillaries with lymphocytic infilteration, demylination in neuronal axons. These changes were more sever at group 4 with presence of different stages of apoptosis in some neurons at group 4. Conclusion: bee venom cannot reduce the harmful effects of hydrogen peroxide on nervous system beside that bee venom it self was harmful on the nervous system of adult male rats at the density of stings used in this study.

Effect of apple vinegar treatment on life variables related to renal function in male rats

Intesar Taha

Journal of Education and Science, 2006, Volume 18, Issue 1, Pages 57-65
DOI: 10.33899/edusj.2006.78657

The research involves the study of effect of apple vinegar on some of biochemical parameters which are related to renal function in experimental male mice of Swiss type. Their weight ranged from (25-30) gms.
The mice .were divided into two groups. The first group was treated with distilled water (control). The second group was treated with diluted apple vinegar by rate 1 water : 3 Apple vinegar by 5ml/kg of body weight . The solution was orally (once daily) administered for three weeks after first, second , and third week of administration blood samples were obtained from the eye-socket. Spectrophometric method was used to determine the biochemical parameters the results showed that (dil) apple vinegar decreased the level of protein, albumin & globulin and decreased the level of uric acid, urea & creatinine in the blood.