Print ISSN: 1812-125X

Online ISSN: 2664-2530

Keywords : Pathogenic bacteria

Resistance of Some Bacteria Isolated from Urinary Tract Infections in Elderly Patients to Antibiotics and Detection of β-lactamases in Them

Mohammed A. Mahmood; Shaker G. Gergees; Alaa T. Younis

JOURNAL OF EDUCATION AND SCIENCE, 2021, Volume 30, Issue 3, Pages 198-208
DOI: 10.33899/edusj.2021.168648

Urinary tract infection is the second-largest infection, especially in elderly people, and the increasing use of antibiotics has led to the emergence and development of resistance in isolated and other bacteria in this study Fifty urine samples from elderly patients with urinary tract infections were collected from both sexes in Mosul city. Twenty-six bacterial isolates were isolated and diagnosed by ordinary bacteriological tests. The isolates were related to the species. Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis. After isolation, their resistance to 14 selected antimicrobial agents was tested by the disk diffusion method. The presence of β-lactamases, in general, was detected by acidimetric method, the detection of extended-spectrum β-lactamases(ESBLs) was performed by double disk method, while the detection of AmpC β-lactamases was performed by cefoxitin resistance method. The isolates showed clear resistance         to β-lactam antibiotics ranged (50%--92.3%),and to ciprofloxacin, gentamicin, sulfamethoxazole, streptomycin, rifampin with rates; (53.8%),(57.7%), (61.5%), (80.8%), (88.5%), respectively, while they showed reduced resistance to amikacin (15.4%). β-lactamases in general were detected in (96.1%) of the isolates, while (19.2%) of them gave a positive result for the detection of (ESBLs). AmpC β-lactamases were detected in (69.2%) of the isolates as they showed a clear resistance to cefoxitin which indicates that they are potential producers of these enzymes.

Antimicrobial Activity of Some Complexes of Zr (IV) and Cd (II) with Benzaldazine Derivatives on Growth of Some Pathogenic Bacteria

Nahida S. Al-Chalabi; Najwa Al-Barhawi; Zohour F. Daood

JOURNAL OF EDUCATION AND SCIENCE, 2021, Volume 30, Issue 3, Pages 63-72
DOI: 10.33899/edusj.2021.168655

 In order to understand the medical effects of heavy metals, the antimicrobial effect of Cd (II) metal ion, Zr (IV) metal ion, Ligand and their complex compounds resulting from their combination, was studied on the growth of gram negative and positive bacteria as well as yeast. It turns out, that all bacterial isolates (Bacillus subtilus, Stapphylococcus aureus and Pseudomonas aeuroginosa, Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Shigella flexneri and the yeast Candida albicans), have a high resistance of 100% to Colistin (10µg/disc) and Nystatin (10µg/disc), while the Zr (IV) metal is better than Cd metal in its effect on inhibiting the growth of different types of pathogenic bacteria isolated in this study.In the study of minimum inhibition concentration (MIC) of metal Cd (II), Zr (IV) and,  Ligand complexes, on the growth of bacterial isolates, it was also shown that Cd and Ligand 4-bromobenzaldazine (BrA) at the concentration 0.00025g/ml, had an effect of 88 and 100% as the lowest inhibitory concentration for the growth of all bacterial isolates of different species. 

Studying the inhibitory activity of the plant and honey seder towards some pathogenic bacteria

Angham Jabbar Alwan; Bushra Deli Hamad; Mohsin Ayoub Issa

JOURNAL OF EDUCATION AND SCIENCE, 2011, Volume 24, Issue 3, Pages 64-76
DOI: 10.33899/edusj.1999.58790

this study was Conducted to estimate the antibacterial activity of the Ziziphus leaves and honey compared with the activity of anumber of antibiotics and other types of honey, the aqueous extract and alcoholic extract were prepared.
The results showed that the aqueous extract of the Ziziphus leaves was more effective than the alcoholic extract of the plant on the bacteria studied.
It was found that increasing concentration of extract was accompanied by an increase in the effectiveness of inhibitory activity and the concentration 200 mg/cm3 had the best inhibitory effect on all bacteria studied Regarding the impact of Ziziphus honey was found to be effective inhibitory effect than in other species, and 100 mg/cm3 was the most effective.
When comparing the effectiveness of the Ziziphus extracts and honey with some antibiotics it was found that the concentration of 200 mg/cm3 of aqueous extract of Ziziphus leaves may have superior the effect on the bacteria studied compared to antibiotics Tetracycline, Rifampin, Doxycycline, Gentamicin. And was comparable in its effect of other antibiotics, Ziziphus honey Display superior to most antibiotics studied depending on the type of bacteria studied.