Print ISSN: 1812-125X

Online ISSN: 2664-2530

Main Subjects : Microbiology

Antimicrobial Activity of Local Rhizobial Isolates Against Some fungi

Omar Hammad Jumaah; Raad H. Sultan; Mahde Saleh Assafi

Journal of Education and Science, 2022, Volume 31, Issue 2, Pages 141-149
DOI: 10.33899/edusj.2022.133511.1230

In this study, three isolates of Ensifer fredii bv. fredii were isolated from root nodules of Vigna unguiculata L. (Cowpea) which collected from different cultural areas in Nineveh Governorate/Iraq. The rhizobial colonies were purified and their agronomic and biochemical characteristics were studied. Microscopic examination results showed rod negative Gram stain bacteria, whereas the three isolates showed ability to grow on rhizobial minimal medium (RMM), motility on TY semi-solid medium, gelatin liquefaction, citrate utilization as a sole carbon source, as well as the isolates showed a positive result for Triple Sugar Iron (TSI). Antimicrobial activity study of local rhizobial isolates against fungi showed a clear effect on studied fungi. The filtrate culture of Ensifer fredii bv. fredii ORM1 showed inhibition zone effect with average 12.6, 10.3 and 12.0 mm against Aspergillus niger MR1, Fusarium solani MR2 and Penicillium spp. MR3, respectively, whereas the filtrate culture of Ensifer fredii bv. fredii ORM13 showed inhibition zone effect with average 10.0, 13.3 and 13.0 mm against Aspergillus niger MR1, Fusarium solani MR2 and Penicillium spp. MR3, respectively. A filtrate culture of Ensifer fredii bv. fredii ORM23 showed less effect against each of Aspergillus niger MR1 and Penicillium spp. MR3 which inhibition zone average 8.6 mm, whereas there is no inhibition effect were recorded for culture filtrate against Fusarium solani MR2.

Estimating the Level of Some Inflammatory Cytokines in the Serum of Women Exposed to Abortion and the Relationship with Toxoplasmosis

Raqaa Alubaidi; Adeeba Shareef

Journal of Education and Science, 2021, Volume 30, Issue 2, Pages 33-41
DOI: 10.33899/edusj.2020.128415.1113

This study includes the collection of (90) blood samples, eighty samples belongs to patients experienced to spontaneous or recurrent abortions, and the ten samples from women with normal pregnancy. The results showed a significant increase in the levels of cytokines in patients serum as their concentrations were (218.15±105.1 pg/L for IL-1β, 63.10±35.17 ng/L for TNF-α and 31.77±25.3ng/ml for IFN-g) compared to the control as it(151.0±0.76 pg/L ,34.96±0.42 ng/L and 11.15±3.49 ng/ml) for the cytokines respectively. The level of cytokines reached (352.18 pg/L,117.8 ng/L and 63.8 ng/ml) for IL-1β,TNF-α and IFN-g respectively in women infected with Toxoplasmosis compared to non-infected women (337.1 pg/L , 101.3 ng/L and 54.06 ng/ml). This indicate that toxoplasmosis has a role in the induction of immune system.

Detection of Bacterial Contamination and Antibiotic Resistance at Neonate Intensive Care Units in Ibn Al-Atheer Teaching Hospital for Children

sahira adrees AL-sanjary; Fulla qaydar al-abas

Journal of Education and Science, 2021, Volume 30, Issue 2, Pages 121-127
DOI: 10.33899/edusj.2021.129017.1123

The study included the isolation and diagnosis of some gram positive and negative bacterial species from (NICU ) in Ibn Al-Atheer Teaching hospital for children from from August 2019 to February 2020 .A total of 90 swabs were obtained from various sites of Intensive Care Unit from Ibn Al-Atheer hospital environment and inoculated on culture media. The obtained growth revealed different bacterial colonies which had been tested for their morphological and biochemical characteristics. Eighty pure isolates were obtained including (76 ) Gram positive, and (4 ) Gram negative bacterial isolates. The highest rate of bacterial contamination had been found in the couh and incubator. The most prevalent bacteria isolated from inanimate surfaces were Staphylococcus aureus, Coagulase negative staphylococci, Bacillus, in addition to Diphtheroids, Lactobacillus, Streptococcus pneumoniae, Klebsiella pneumoniae, and E, coli.
Antibiotic susceptibility testing for all isolates was performed using (8) types of commonly used antibiotics in Iraq. Taken all together, gram positive and negaive bacteria showed high resistance against streptomycin, erythromycin, and ampicillin respectively. Notably, resistance to ampicillin and gentamicin were demonstrated which are commonly given as the first line of treatment in NICUs.

Pathophysiological consequences of SARS CoV-2

Hadeel Mohammad Hameed; hiyam natheer maty; fanar ablahad isihak

Journal of Education and Science, 2021, Volume 30, Issue 1, Pages 24-33
DOI: 10.33899/edusj.2020.127225.1089

Coronavirus outbreak occurred in late 2019 and called (COVID-19), caused by SARS-CoV-2 which firstly emerged in Wuhan city/China. This virus mostly attacks the respiratory system and therefore is more likely to destroy lung tissue and cause pneumonia; although lung is the target tissue but many organs of COVID 19 patients affect by virus invasion and showed numerous Physiological consequences. Angiotensin converting enzyme-2 (ACE2) receptors for the virus attachment are distributed in human's tissues including “lungs, heart, kidneys, intestines, brain and testes” that are recognized as a possible targets of COVID-19.The vital functions of these organs may impaired with different levels as a result of viral infection and replication. Patient with type A especially those formerly have been identified with cardiovascular diseases in particular increased blood pressure, are more expected to develop acute COVID-19 symptoms. Clot producing disorders with SARS CoV-2 infected patients were observed firstly by researchers in China. Thus untreated patients revealed large arterial pulmonary clots can put permanent pressure on the heart, leading to cardiac attack. Anxiety about the coronavirus outbreak is extremely common. Therefore, some individual may showed a huge concern that can disturb their regular life. Stress and fear from infection can be devastating and cause powerful excitements in children and adults. Therefore this study aims to shed light on pathophysiological consequences and stressful conditions of COVID-19 in patients including many of boby organs and ABO system.

Incidence and Antibiotic Sensitivity of Klebsiella pneumonia isolated from urinary tract infection patients in Zakho emergency hospital / Iraq

Reem F Polse; Sozan M Qarani; Mahde Saleh Assafi; Nisreen Sabaly; Fawaz Ali

Journal of Education and Science, 2020, Volume 29, Issue 3, Pages 257-268
DOI: 10.33899/edusj.2020.126827.1056

Uropathogenic Klebsiella pneumoniae is considered the most important causes of urinary tract infection, and recently the antibiotic resistance of this pathogen has increased dramatically. The study aims at investigating the prevalence and antibiotic sensitivity of uropathogenic K. pneumoniae among different ages of groups at both genders. 1737 urine specimens were collected from individuals admitted to Zakho emergency hospital from January 2016 until December 2018. K. pneumoniae was identified based on Gram stain, colony characteristics and biochemical tests, and then it was tested for their antibiotic sensitivity. Out of all samples, 1076 (61.9%) showed significant bacterial yield. The UTIs among females (64.3%) were statistically higher than males (46.5%) (P<0.001). The age group of 20-29 years was highly susceptible to UTIs (43%). Among the total UTIs, K. pneumoniae represented 16.2%. K. pneumoniae in males (23.4%)was significantly higher than females (15.4%) (P=0.03).The age group 40-49 years (32.2%) was the highest group that at risk for K. pneumoniae infection. K. pneumoniae was highly susceptible to imipenem (100%) and was highly resistant against cephalothin (90.8%). A high prevalence of UTIs were observed among the age group of 20-29 years and the females had more risk of having UTIs. The males were more susceptible to K. pneumoniae with higher prevalence in the age group of 40-49 years. K. pneumoniae showed high sensitivity toward imipenem and high resistance to cephalothin. More awareness is required in all healthcare sectors for regular surveillance of the use of antimicrobial agents to reduce the resistance rates and to eradicate the pathogens.

Isolation and Diagnosis of Bacterial Infections Among Children in the West Bank of Mosul City


Journal of Education and Science, 2020, Volume 29, Issue 2, Pages 216-229
DOI: 10.33899/edusj.2019.125919.1005

Bacterial infections are common in neonates, infants and children.. Some of these infections are serious and usually carry high risk of fatal complications such as septicemia / meningitis if left un-treated. Diagnosis of such infections is usually clinical together with the identification of bacteria in body fluids such as urine, stool, blood and CSF. The aim of the current study is to isolate and identify bacteria responsible for some types of serious bacterial infection in neonates such as UTI, GIT infections and bacteremia in West bank of Mosul city sand test their susceptibility to different antibiotics. Our results indicated that Staphylococcus aureus was the most common bacterial isolate in blood (81%), whereas both Staphylococcus aureus and E. coli were common in urine (48% and 43% respectively). These microorganisms are highly sensitive to amoxiclav (83% for Staph , and 100% for E.coli) , levofloxavin ( 88% for Staph. and 100 % for E.coli) and meropenem (100% for both Staph and E.coli). However, both of them highly resistant to ampicillins (100% resistant rate) and 3rd generation cephalosporins. Although meropenem is effective, its use should be selected and restricted to highly resistant cases to avoid the emergence of early antibiotic resistance.

Assessment of Fungal Growth at Hemodialysis and Peritoneal Dialysis Unit in Hawler Teaching Hospital

Vian Badraddin Al-Barzinji

Journal of Education and Science, 2020, Volume 29, Issue 2, Pages 279-286
DOI: 10.33899/edusj.2020.126676.1049

Dialysis is used when the patient's kidneys can no longer perform their functions normally; it is a treatment that performs the functions of natural kidneys. Most patients begin dialysis when their kidneys have lost 85-90% of their ability to work naturally and they have to depend on dialysis for the rest of their lives, is called end –stage renal disease (ESRD). Infection in patients with end stage renal disease (ESRD) is usually treated with hemodialysis (HD) or peritoneal dialysis (PD). End stage renal disease a major cause of morbidity and mortality, in order to assess the fungal growth at hemodialysis and peritoneal dialysis unit a descriptive study was conducted at hemodialysis and peritoneal dialysis units in Hawler teaching hospital, from 15th of January to the end of May 2017;the study also includes the access sites and peritoneal catheter site. Factors that affected the access site among dialysis patient in the hospital. Sample of 63 patients, who were attending to the dialysis unit at time of the data collection, had been taken; in addition to that swab samples had been gathered from access site and peritoneal catheter site. The results of the study revealed that the fungal growth was positive at dialysis access sites, where the total percentage was 84.1% and that of peritoneal catheter site was 15.85%. In these unites, different types of filamentous fungi and yeasts had been successfully isolated.