Study the inhibitory effect of mother's milk and its comparison with colostrum in isolates of Candida albicans isolated from patients with oral candidiasis in Mosul city and their effect on phospholipase production
JOURNAL OF EDUCATION AND SCIENCE,
2018, Volume 27, Issue 2, Pages 75-94
AbstractThe current study was included isolation and identification of the yeast Candida albicans from patients infected with oral candidiasis. Thirty six yeast isolates were obtained grown on Sabouraud's dextrose agar medium (SDA) , the colony colour was from white to creamy and it was smooth with curved surface . Symbols were given to isolates from RA1 to RA36 in order to differentiate them. Identification by Vitek 2 Compact system showed that 29 isolates belonging to the species C. albicans . This study included the evaluation of phospholipase enzyme production on egg yolk agar for all C. albicans isolates and the results showed that 23 isolates (79.31%) were producers of the enzyme and precipitation zone (Opaque zone) was observed around the grown colonies with different precipitation zones. Evaluation of the effect of colostrum and mother's milk was carried out on growth of 10 isolates of C. albicans exhibiting highest activity in its production of enzyme. There wasn't any inhibitory effect noted on the isolates understudy at the concentrations 1, 2, 4, 8, 16 and 32% of colostrum or milk by agar dilution – streak assay and also by disk diffusion method at the concentration 100% , but the effect on phospholipase production was observed of colostrum at the concentration 32% on three isolates , namely RA4, RA6 and RA13 (30%), whose production was reduced The test also showed that the effect of milk at the concentration 32% reduced the production of isolates RA4 and RA13 (20%) .
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