Indirect Spectrophotometric Determination of Niclosamide in Pharmaceutical Preparations
JOURNAL OF EDUCATION AND SCIENCE,
2018, Volume 27, Issue 3, Pages 1-18
AbstractA simple and sensitive indirect spectrophotometric method is developed for the determination of niclosamide in pharmaceutical preparations. The method is based on the oxidation of reduced form of niclosamide with iron ( III ) in acidic medium, then the subsequent reaction of iron ( II ) with 1,10- phenanthroline to produce a ferroin complex which is water - soluble , stable (using EDTA solution improve the stability of the complex) , and has a maximum absorption at 510 nm against the reagent blank with a molar absorptivity of 2.19×104 l.mol-1.cm-1 . Beer,s law is valid over the concentration range of 2.5 to 225 μg/10 ml. The relative error is in between - 1.80 and +1.50 % and the relative standard deviation from ± 0.89 to ± 2.07 % depending on the concentration of niclosamide . The proposed method has been applied successfully to determine niclosamide in pharmaceutical preparations.
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