Determination of Mesalazine Spectrophotometry Based on The Charge Transfer Complex n- π Using Reagent p-bromanil
JOURNAL OF EDUCATION AND SCIENCE,
2019, Volume 28, Issue 2, Pages 71-84
AbstractSimple, sensitive and rapid spectrophotometric method for the determination of mesalazine in pure form and pharmaceutical preparations is described. The method is based on the reaction of mesalazine with p-bromanil in the presence of borate buffer solution of pH9 to form a pink color charge transfer complex of maximum absorption peak (λmax) at 346 nm. Under the optimized reaction conditions, Beer’s law correlating the absorbance with mesalazine concentration was obeyed in the range of 0.48-12 μg ml-1. The molar absorptivity was 6.5×103 L.mol−1cm−1. The limits of detection was 0.053μg ml-1. The accuracy and precision of the method were satisfactory; the average recovery was 98.04% and values of relative standard deviations better than 1.70 %. The stoichiometry of the reaction was studied, and the reaction mechanism was postulated. The proposed method was successfully applied to the determination of mesalazine in its pharmaceutical tablet and capsule with good accuracy and precisions. The results obtained by the proposed method were compared with those obtained by the official method.
1- Actis.GC, Pllicano.R and Rizzetto.M, "Individually administered or co-prescribed thiopurines and mesalamine for inflammatory bowel disease" .World J Gastroenterol 15;1420-1426,(2009).
2- Sandborn.WJ, Feagan.BG and Lichtenstein.GR, "Medical management of mild to moderate Crohn's disease : evidence based treatment algorithms for induction and maintenance of remission"; Aliment Pharmacol Ther 26:987,(2007).
3- US National Library of Medicine 8600 Rockville Pike,Bethesda,MD 20894 US Department of Health and Human Service National Institutes of Health Page Last updated May (2018) .
4- Finkel, Cubeddu and Clark; Lippincott's Illustrated Reviews: Pharmacology, 4th Edition; 393,( 2009).
5- Al-Shreify.Z.A.M, "Development of spectrophotometric and fluorophotometric methods for determination of phenolicand nitrogenic drug compounds",M.Sc. Thesis, Mosul University, (2012).
6- Abdalla.F.A.A and Elbashir.A.A, "Development and validation of spectrophotometric methods for the determination of mesalazine in pharmaceutical formulation", J.Med.Chem, 4(3),361-366.,11,(2014).
7- Zakaria.R.A, "Spectrophotometric determination of Mesalazine by
8-hydroxyquinoline and N-(1-naphthyl)ethylene- diaminedihydrochl oride reagents in bulk and capsule dosage forms", J. Sci.,24(1),146-158,(2013).
8- Al-Enzy.M.S, Al-Sabha.T.N and Al-Ghabsha.T.S, "Use of charge transfer complex reaction in spectrophotometric micro Determination of some drugs", Jord. J. Chem.,7,87-102,(2012).
9- Al-Sabha.T.N, Al-Enizzi.M.S and Al-Taee.O.A, "Application of chloranil and fluoranil π- acceptors for the Spectrophotometric determination of mesalamine in pharmaceuticals", Eur. Chem. Bull., 3(4),377-383,(2014).
10- Salih.E.S and Al-Sharook.M.M, "Spectrophotometric assay of mesalazine in pharmaceutical preparation via oxidative coupling reaction with thymol and sodium metaperiodate", J.Edu.&Sci.,21, 103-115,(2008).
11- Al-Fakhry.M.H.A, "The use of oxidative coupling reaction for spectrophotometric determination of aniline and its substituents and the drugs dipyrone and mesalazine", M.Sc.Thesis, Mosul University, 64-79,(2006).
12- Al-Daoudi.N.M.I, "Spectrophotometric determination of some drugs by using prussic blue reaction and methylene blue and rhodamine-B dyes", M.Sc.Thesis, Mosul University,62-119,(2011).
13- Al-Nuaimi. I. K. O, "Development of spectrophotometric and fluoro photometric methods for determination of some drug compounds ,M.Sc.Thesis, Mosul University,(2017).
14- Gui.F.L, Li.W, Fan.Y.C and Hu.Z-D, "Fluorescence spectroscopy studies on 5-aminosalicylic acid and zinc 5-aminosalicylate with human serum albumin", J.Pharm.Biomed.Anal.,39,189- 197,(2004).
15- Al-Abdaly. Z. Z. S, "Development of Spectrophotometric ,Spectro fluorometric and High-performance Liquid ChromatographicMethods for Determination of some Drug Compounds", Ph.D.Thesis, Mosul University,(2013).
16- AL-Meshaekhy.M.Y,״Development of spectrophotometric methods and atomic absorption in drug compounds analysis using different reactions ״,Ph.D. Thesis, Mosul University,(2017).
17- Nigoric.B and Imunic.B, "Determination of 5-amino salicylic acid in pharmaceutical formulation by differential pulse voltammetry", J. Pharm. Biomed. Anal.,31,169-174,(2003).
18- Rao.K.H, Rao.A.L and Sekhar.K.C, "Validated PR-HPLC method for the estimation of mesalazine in bulk and tablet dosage form", Int. J. Res. Pharm. Chem., 3, 472- 480,(2013).
19- Elmasry. M. S, Blagbrough. I. S, Rowa. M. G and Saleh. H. M, "Quantitative HPLC analysis of mebererine, mesalazine, sulphasalazine and dispersible aspirin stored in avenalink monitored dosage system with co-prescribed medicine", J.Pharm. Biomed. Anal.,54, 646-652,(2011).
20- Abdolmohammad- Zadeh. H and Kohansal. S, "Determination of mesalamine by spectrofluorometry in human serum after solid-phase extraction with Ni-AL layered double hydroxide as a nanosorbent", J. Braz. Chem. Soc.,23,473-781,(2012).
21- Pastorini. E, Locatelli. M, Simoni. P, Roda. G and Roda. E, "Development and validation of an HPLC-ESI- MS/MS method for determination of 5-aminosalicylic acid its major- metabolites N-acetyl-5-aminosalicylic acid in human plasma", J. Chromatogr. B, 872, 99- 106,(2008).
22- Rafael.J.A, Jobar.J.R , Gasagrande.R and Geargetti.S.R, "Validation of HPLC, DPPH and nitrosation methods for mesalamine determination in pharmaceutical dosage forms", Braz.J. Pharm. Sci., 43,(2007).
23- Skoog.D.A, West.D.M, Holler.F.J and Crouch.S.R, "Fundamentals of Analytical Chemistry", 9th ed. Mary Finch,USA,(2013).
24- Townshend.A, "Analytical applications of molecular complexes", Proc. Soc. Anal. Chem.,
25- British pharmacopeia, CD-ROM, (2015).
26- Harevy.D, ״Modern Analytical Chemistry״, McGarw-Hill Higher Education,USA,(2000).
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