The antimicrobial effect of Trigonella graecum foenum, Sinorhizobium meliloti and Escherichia coli on the growth of three types of pathogenic bacteria
JOURNAL OF EDUCATION AND SCIENCE,
2018, Volume 27, Issue 1, Pages 53-70
AbstractThe present study investigates the inhibitory effect of the oil extracted from Trigonella foenum-graecum seeds and Exopolysaccharides (EPS) which is isolated from the symbiotic bacteria, namely, Sinorhizobium meliloti and the standard bacteria, i.e. Escherichia coli. The study conducted by utilizing an unified concentration of 200 mg/ml in the growing of three types of pathogenic bacteria which are Escherichia coli and Proteus miribilis and Staphylococcus aureus. The results of the study show that there is a sort of matching between the oil extracted from Trigonella foenum-graecum seeds and the extract Exopolysaccharides (both types) with the utilized standard samples by means of the TLC technique. Similarly, the study testified the chemical structure of these three extracts by using the spectrum infrared rays. It has been shown that the oil extracted from Trigonella foenumgraecum seeds consists of the group O=C-OH and the group C=H aromatic and alkyl. Similarly it also contains the group of acids, aster and thin aromatic. Whereas EPS (with both types) consists of alcohol OH and the carboxyl group C-OH and CH aliphatic in addition to aster and ether compounds and the two groups of carbonyl C=O that belong to Glucuronic and Pyruvate acids. All these compounds had a lethal effect on the growth of bacteria.
The results also show the biological activity of the above mentioned extracts. The first, Exopolysaccharides (EPS) that is extracted from the bacteria Sinorhizobium meliloti, comes in the first position with antimicrobial activity on the growth of the pathogenic bacteria Proteus miribilis. Secondly, comes EPS isolated from the bacteria Escherichia coli, then comes the oil extracted from Trigonella foenum-graecum seeds especially on the growth of Proteus miribilis and Staphylococcus aureus. These results were registered with a significant value estimated to 0.0627 and 0.2453 respectively compared with a comparative sample at significant degree (p≤0.05).Whereas the bacteria Escherichia coli showed a weak sensitivity against the third extract compared with the first and second extracts.
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