Mice Corneal Damage Mediated by Pseudomonas aeruginosa Extrcellular Proteases
JOURNAL OF EDUCATION AND SCIENCE,
Volume 20, Issue 2, Pages 22-28
Extra cellular Proteases of Pseudomonas aeruginose had been considered to be one of the important virulence factors in corneal infections. · Production of these enzymes from strains that elicited corneal damages in mice were detected in approximately 12 % of 25 isolates obtained from AI- Zahrawii hospital in Mosul. Intra -corneal injection of neonates 5 days old with the crude proteases preparation cause death most of them within 24 h . The injection of these preparation intra corneally into 30 days old mice elicited severe damages that grossly appeared as a white spot within 4-5 days at the site of injection. While the injection of partially purified proteases produced the same corneal damage which was identified grossly within 24h only. This revealed the importance of these enzymes as a virulence factor for these bacteria in corneal infection.
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