Effect of aquatic extracts of oleander and Melia azedarach on the nucleic acid content of trichomonas vaginalis in vivo
JOURNAL OF EDUCATION AND SCIENCE,
Volume 20, Issue 1, Pages 22-31
AbstractThis study was conducted to elucidate the effect of aqueous leaf extract
of Nerium oleander and fruit extract of Melia azedarach on nucleic acids
content and activity of some enzymes in Trichomonas vagina/is which
growth in Diamond's medium tryptone, yeast and maltose (TYM) media.
Results indicated that the exposure of T. vagina/is to IC50
concentration of N. oleander leaves and M azedarach fruits extracts
showed asignificant decrease in DNA content by 31.5% and 38.2%,
respectively, while the RNA content decreased by 28.3% and 50.7%,
respectively. Furthermore the totel.l. nucleic acids content decreased by
27.9% and 48.1% , respectively, compared with the untreated
trichomonads with plant extracts after 72 hours of growth.
The results of this study also indicated that T. vagina/is treated with
N. oleander leaves and M azedarach fruits extracts showed inhibed in the
activity of thymidine phosphorylase enzyme at rate 32.7% and 69.2%
respectively compared with untreated trichomonads with plant extracts
after 72 hours of growth.
From the results of this study, 1t is obvious that the above mentioned
extracts possess an inhibitory effect on nucleic acids and on the activity
of the enzyme Thymidine phosphorylase.
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