Spectrophotometric determination of a number of aliphatic amines in aqueous solution and application to some pharmaceutical preparations
JOURNAL OF EDUCATION AND SCIENCE,
2013, Volume 26, Issue 5, Pages 17-32
A simple , rapid and sensitive spectrophotometric method for the determination of aliphatic amines was developed .The method is based on the proton transfer reaction using 3,5- dinitrosalicylic acid (DNS) reagent in basic medium forming colored products with maximum absorption at 415 nm .The molar absorptivity ranged from 5100 l. mol-1 .cm-1 for tributylamine to 12000 l. mol-1.cm-1 for dibutylamine .The method is obeyed Beer’s law over the concentration range (0.12-12) µg.ml-1.The accuracy ( average recovery ) is 99.73% and the precision (RSD) is less than 4.50% .This method is applied successfully for the assay of ethanolamine and ethylenediamine in their pharmaceutical preparations .
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