Comparison of Methods for Detection of Chlamydia trachomatis in Cervical from Women in Mosul City
JOURNAL OF EDUCATION AND SCIENCE,
2020, Volume 29, Issue 1, Pages 121-134
AbstractThe study included detection of Chlamydia trachomatis in the cervix of symptomatic and a symptomatic woman.
A total of (60) woman aged between (16-<45) years were diagnosed by the consultant of the general hospital in Mosul city during the period May until the end of December 2013.
Three endocervical swabs were collected from each woman. One of the three swabs was stained with Giemsa stain for direct examination by light microscope, and the other swabs was put in 0.2M sucrose phosphates transport media then inoculated in yolk Sac of embryonated chicken eggs, and the last endocervical swabs was used for detection of chlamydia by using rapid immunochromatographic card test . From each woman participated in this study 5 ml of venous blood was collected to detect the immunoglobulin (IgG) by ELISA and indirect microimmuno flourescence test.
The result showed that C.trachomatis was presented in percentage (35%) by Indirect microimmunoflourescence test (30%) by ELISA (25%) by culturing (21.6) by Giemsa stain, , (5%) by rapid test in symptomatic and asymptomatic women.
This result showd that Indirect MicroimmunoaFlourecence is more specific to detect Chlamydia than other test while the rapid test was of no value in detection of chlamydia in endocervical swabs
Also the result showed that the percentage of Chlamydia in a asymptomatic woman such as infertility and abortion had high percentage than symptomatic woman such as vaginal discharge, abdominal pain' and ectopic pregnancy . Also the result showed that the isolation rate in age group (21-30) years was the more dominate than other age group.
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