Kinetic and inhibitory study of partially purified lipoxygenase from epilepsy patients serum
JOURNAL OF EDUCATION AND SCIENCE,
2021, Volume 30, Issue 1, Pages 58-71
This research was included partially purification of lipoxygenase (LOX) from serum of patients with epilepsy using ammonium sulfate precipitation, dialysis and ion-exchange chromatography with specific activity (0.751 U / mg) , (0.935 U/mg) and (2.60 U/mg) respectively . Optimum conditions for enzyme activity were determined. The best enzyme activity was showed at 7 minutes of incubation time , 3 minutes of reaction time, pH = 7, temperature 40Cº and finally substrate concentration (linoleic acid) was (1.2 mmol/L) . By applying Michaelis-Menten and Lineweaver-Burk Equations, Michaelis-Menten constant (km) and Vmax values were found 0.3 (mM) and (0.9 U/ml) respectively. Also this study included the effect of some antiepileptic drugs such as valproic acid , carbamazepine and acetozolamide. Acetazolamide showed the highest inhibition of lipooxygenase activity(96.2٪) , valproic acid (95.6) and Carbamazapine (95.1٪). Inhibition type was studied and the result showed noncompetitive inhibition by using Lineweaver-Burke plot for all above drugs.
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