Journal of Education and Science

Abstract

The results of a field survey of some potato growing areas in Nineveh Governorate showed the presence of Potato virus s PVS, which was detected by the Double Antibody Sandwich Enzyme-Linked ImmunoSorbent Assay DAS-ELISA test.
This record is the first in the governorate. Through the absorbance readings using the elisa reader, highest value was recorded in the Sherikhan area samples, which amounted to 1.190 nm, while lowest value was recorded in the Rabia area samples with a value of 0.756 nm, while absorbance value of the negative standard sample was 0.300 nm. The frequency of virus in the Sherikhan area was 53.3%, while frequency of the virus in the Rabia area was 28.8%.
As a result of mechanical inoculation with virus isolates diagnosed by DAS-ELISA test, spread of the common strain PVSO through symptoms on Chenopodium quinoa, which was in the form of small chlorotic spots on the inoculated leaves, and new leaves were devoid of any disease symptoms.