Effect of some growth regulators on initiation, growth and differentiation of Dianthus caryophyllus L. callus.
JOURNAL OF EDUCATION AND SCIENCE,
2013, Volume 26, Issue 4, Pages 74-88
The current study evaluated the effect of some growth regulators on initiation, growth and differentiation of (Dianthus caryophyllus L.) callus.The results showed that callus could be initiated when (BA,IAA) and (BA,NAA) added to MS medium. Variation in callus initiation were recorded according to the source from which the explants took which were (stem, leaves, cotyledons, roots and hypocotyls) it was shown that the best initiation of callus took place on MS1 (MS +BA 0.1mg/L+ 0.5 2,4-D mg/L) and MS2 (MS+BA 0.1mg/L + IAA0.1mg/L).
Callus derived from cotyledons and leaves exhibit differentiation the best ratios for shoot formation from cotyledons leaves was MS(BA 1.0 mg/l +IAA 0.05mg/l) and (MS + BA 3.0mg/l + IAA0.1mg/l) for callus derived from leaves. results showed that use the auxins IBA, NAA, and IBA enhanced rooting of the regenerated shoots. Rooting percentage reached 100%. when 1.0 mg/l of IBA added to MS medium. the rooted plants were successfully acclimatized and transferred to the soil.
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