Abstract
We achieved an economical conclusion for Laboratory propagation of gladiolus bulbs Gladiolus grandiflorus using tissue culture techniques. This method include the use of corm slices with 8-10 millimeters thickness which were lied in contact with Murashige and Skoog solid medium supported with 0.25, 0.5, 1.0 and 2.0 mg/L of NAA or 2,4-D interaction with different concentrations (0.5, 1.0, 2.0 and 4.0 mg/L) of BA and Kinetin. The number of shoots obtained at the level of 4.0 mg/L BA combined with 1.0 mg/L of NAA was 5.1 folds while the largest number of shoots 4.9 folds were found with the kin at 4.0 mg/L with 1.0 mg/L of NAA. The later has more impact on vegetative growth and the number of shoots formed than 2,4-D.
The sucrose and NAA had a great effect on the rooting of shoots and formation of corms, through the best periods of time for each of them. Rooting were 100% when used 60 g/L sucrose within 14 days, and took 56 days to produce corms.
When 90 g/L of sucrose were used with 10 mg/L NAA for rooting, shoots were rooted within 11 days and corm formation 7.0 corm/shoot were accomplished after 42 days. The corms were ready for planting in the filed.
