الملخص

Abstract The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml. The apparent molecular weight of leptin had been determined by gel filtration chromatography technique using Sephadex G - 75, and by SDS electrophoresis, the results showed that the apparent molecular weight for the band number 5 of leptin hormone was 15750353 Dalton and 16000 Dalton respectively.