Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques

Abstract

Abstract The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml. The apparent molecular weight of leptin had been determined by gel filtration chromatography technique using Sephadex G - 75, and by SDS electrophoresis, the results showed that the apparent molecular weight for the band number 5 of leptin hormone was 15750353 Dalton and 16000 Dalton respectively.